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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | upstream transcription factor 1 |
| Gene Symbol: | USF1 |
| Synonyms: | UEF; FCHL; MLTF; FCHL1; MLTFI; HYPLIP1; bHLHb11; USF1 |
| Gene ID: | 7391 |
| UniProt ID: | P22415 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human USF1 (NP_009053.1). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.1 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-USF1 antibody |
Background
This gene encodes a member of the basic helix-loop-helix leucine zipper family, and can function as a cellular transcription factor. The encoded protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. This gene has been linked to familial combined hyperlipidemia (FCHL). Alternative splicing of this gene results in multiple transcript variants. A related pseudogene has been defined on chromosome 21.
Images
 | Western blot analysis of various lysates, using USF1 Rabbit pAb (A13560) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using USF1 Rabbit pAb (A13560) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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