VGF Rabbit pAb (20 μl)

VGF Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14795
Availability: In Stock
$108.00
-+
Reactivity:   Rat    
Applications:   WB, ELISA    
Host Species:   Rabbit    
Isotype:   IgG    
Clonality:   Polyclonal antibody    
Gene Name:   VGF nerve growth factor inducible
Gene Symbol:   VGF
Synonyms:   SCG7; SgVII; VGF
Gene ID:   7425
UniProt ID:   O15240
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 466-615 of human VGF (NP_003369.2).
Dilution:   WB 1:500-1:2000
Purification Method:   Affinity purification
Concentration:   1.08 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-VGF antibody




Background

This gene is specifically expressed in a subpopulation of neuroendocrine cells, and is upregulated by nerve growth factor. The structural organization of this gene is similar to that of the rat gene, and both the translated and the untranslated regions show a high degree of sequence similarity to the rat gene. The encoded secretory protein also shares similarities with the secretogranin/chromogranin family, however, its exact function is not known.




Images

VGF antibodyWestern blot analysis of lysates from rat brain, using VGF Rabbit pAb (A14795) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.



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