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| Reactivity: | Human |
| Applications: | WB, IHC, IF/IC, IP, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | X-ray repair cross complementing 5 |
| Gene Symbol: | XRCC5 |
| Synonyms: | KU80; KUB2; Ku86; NFIV; KARP1; KARP-1; Ku80 |
| Gene ID: | 7520 |
| UniProt ID: | P13010 |
| Clone ID: | 7N7G3 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 633-732 of human Ku80 (P13010). |
| Dilution: | WB 1:1000-1:6000; IHC 1:200-1:2000; IF/IC 1:100-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.25 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-XRCC5 antibody |
Background
The protein encoded by this gene is the 80-kilodalton subunit of the Ku heterodimer protein which is also known as ATP-dependant DNA helicase II or DNA repair protein XRCC5. Ku is the DNA-binding component of the DNA-dependent protein kinase, and it functions together with the DNA ligase IV-XRCC4 complex in the repair of DNA double-strand break by non-homologous end joining and the completion of V(D)J recombination events. This gene functionally complements Chinese hamster xrs-6, a mutant defective in DNA double-strand break repair and in ability to undergo V(D)J recombination. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity.
Images
 | Western blot analysis of various lysates, using Ku80 Rabbit mAb (A12338) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophageal using Ku80 Rabbit mAb (A12338) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Confocal imaging of HeLa cells using Ku80 Rabbit mAb (A12338,dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 60x. |
 | Immunoprecipitation analysis of 300 μg extracts of HeLa cells using 3 μg Ku80 antibody (A12338). Western blot was performed from the immunoprecipitate using Ku80 antibody (A12338) at a dilution of 1:1000. |
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