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| Reactivity: | Human, Mouse |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | RB transcriptional corepressor like 2 |
| Gene Symbol: | RBL2 |
| Synonyms: | Rb2; P130; BRUWAG; RBL2 |
| Gene ID: | 5934 |
| UniProt ID: | Q08999 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 413-613 of human RBL2 (NP_005602.3). |
| Dilution: | WB 1:500-1:1000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 2.345 mg/ml |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RBL2 antibody |
Background
Enables promoter-specific chromatin binding activity. Involved in regulation of lipid kinase activity. Acts upstream of or within negative regulation of gene expression. Located in chromosome; cytosol; and nuclear lumen.
Images
 | Western blot analysis of lysates from Neuro-2a cells, using RBL2 Rabbit pAb (A13649) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 120s. |
 | Immunofluorescence analysis of U2OS cells using RBL2 Rabbit pAb (A13649) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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