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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | RNA sensor RIG-I |
| Gene Symbol: | RIGI |
| Synonyms: | RIG1; DDX58; RIG-I; RLR-1; SGMRT2; Rig-I/DDX58 |
| Gene ID: | 23586 |
| UniProt ID: | O95786 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 726-925 of human Rig-I/DDX58 (NP_055129.2). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.26 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RIGI antibody |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of the antiviral innate immune response. Mutations in this gene are associated with Singleton-Merten syndrome 2.
Images
 | Western blot analysis of various lysates using Rig-I/DDX58 Rabbit pAb (A13407) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 90s. |
 | Immunofluorescence analysis of H9C2 cells using Rig-I/DDX58 Rabbit pAb (A13407) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using Rig-I/DDX58 Rabbit pAb (A13407) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U20S cells using Rig-I/DDX58 Rabbit pAb (A13407) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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