C18orf8 Rabbit pAb (20 μl)

C18orf8 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A15825
Availability: In Stock
$108.00
-+
Reactivity:   Human, Mouse, Rat    
Applications:   WB, IHC, IF/IC, ELISA    
Host Species:   Rabbit    
Clonality:   Polyclonal antibody    
Gene Name:   regulator of MON1-CCZ1
Gene Symbol:   RMC1
Synonyms:   MIC1; Mic-1; WDR98; C18orf8; HsT2591
Gene ID:   29919
UniProt ID:   Q96DM3
Immunogen:   Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human C18orf8 (NP_037458.3).
Dilution:   WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method:   Affinity purification
Concentration:   2.16 mg/ml
Buffer:   Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
Storage:   Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-RMC1 antibody




Background

This gene encodes a colon cancer associated protein.




Images

RMC1 antibodyWestern blot analysis of various lysates using C18orf8 Rabbit pAb (A15825) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney using C18orf8 Rabbit pAb (A15825) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat kidney using C18orf8 Rabbit pAb (A15825) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using C18orf8 Rabbit pAb (A15825) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.
Immunofluorescence analysis of L929 cells using C18orf8 Rabbit pAb (A15825) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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