RPE Rabbit pAb (20 μl)

RPE Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A14778
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: ribulose-5-phosphate-3-epimerase
Gene Symbol: RPE
Synonyms: RPE2-1; RPE
Gene ID: 6120
UniProt ID: Q96AT9
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-228 of human RPE (NP_954699.1).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:100
Purification Method: Affinity purification
Concentration: 2.13 mg/ml
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-RPE antibody




Background

Enables metal ion binding activity; protein homodimerization activity; and ribulose-phosphate 3-epimerase activity. Involved in carbohydrate metabolic process and pentose-phosphate shunt. Located in extracellular exosome.




Images

EMD antibodyWestern blot analysis of various lysates using RPE Rabbit pAb (A14778) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
Immunofluorescence analysis of L929 cells using RPE Rabbit pAb (A14778) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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