RPL18 Rabbit pAb (20 μl)

RPL18 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10720
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: ribosomal protein L18
Gene Symbol: RPL18
Synonyms: L18; eL18; DBA18; RPL18
Gene ID: 6141
UniProt ID: Q07020
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-188 of human RPL18 (NP_000970.1).
Dilution: WB 1:500-1:1000
Purification Method: Affinity purification
Concentration: 3.63 mg/ml
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-RPL18 antibody




Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18E family of ribosomal proteins that is a component of the 60S subunit. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.




Images

RPL18 antibodyWestern blot analysis of various lysates using RPL18 Rabbit pAb (A10720) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Western blot analysis of lysates from Rat liver using RPL18 Rabbit pAb (A10720) at 1:3000 dilution incubated overnight at 4℃.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.



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