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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ribosomal protein L24 |
| Gene Symbol: | RPL24 |
| Synonyms: | L24; eL24; HEL-S-310; RPL24 |
| Gene ID: | 6152 |
| UniProt ID: | P83731 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 38-157 of human RPL24 (NP_000977.1). |
| Dilution: | WB 1:500-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.10 mg/ml |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RPL24 antibody |
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L24E family of ribosomal proteins. It is located in the cytoplasm. This gene has been referred to as ribosomal protein L30 because the encoded protein shares amino acid identity with the L30 ribosomal proteins from S. cerevisiae; however, its official name is ribosomal protein L24. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Images
 | Western blot analysis of various lysates using RPL24 Rabbit pAb (A14255) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates / proteins: 25 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
 | Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using RPL24 Rabbit pAb (A14255) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RPL24 Rabbit pAb (A14255) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of L929 cells using RPL24 Rabbit pAb (A14255) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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