RPS20 Rabbit pAb (20 μl)

RPS20 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10363
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IF/IC, ELISA    
Host Species: Rabbit    
Isotype: IgG    
Clonality: Polyclonal antibody    
Gene Name: ribosomal protein S20
Gene Symbol: RPS20
Synonyms: S20; uS10; RPS20
Gene ID: 6224
UniProt ID: P60866
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-119 of human RPS20 (NP_001014.1).
Dilution: WB 1:500-1:2000; IF/IC 1:50-1:200
Purification Method: Affinity purification
Concentration: 2.23 mg/ml
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-RPS20 antibody




Background

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10P family of ribosomal proteins. It is located in the cytoplasm. This gene is co-transcribed with the small nucleolar RNA gene U54, which is located in its second intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. Two transcript variants encoding different isoforms have been identified for this gene.




Images

RPS20 antibodyWestern blot analysis of various lysates using RPS20 Rabbit pAb (A10363) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
Immunofluorescence analysis of L929 cells using RPS20 Rabbit pAb (A10363) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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