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p90Rsk/RSK1/RPS6KA1 Rabbit pAb (20 μl)
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | ribosomal protein S6 kinase A1 |
| Gene Symbol: | RPS6KA1 |
| Synonyms: | RSK; HU-1; RSK1; p90Rsk; MAPKAPK1; MAPKAPK1A; p90Rsk/RSK1/RPS6KA1 |
| Gene ID: | 6195 |
| UniProt ID: | Q15418 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of human p90Rsk/RSK1/RPS6KA1 (NP_002944.2). |
| Dilution: | WB 1:500-1:2000; IHC 1:100-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.99 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RPS6KA1 antibody |
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 nonidentical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Images
 | Western blot analysis of various lysates using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (A15718) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse spleen using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (A15718) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunofluorescence analysis of L929 cells using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (A15718) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2 OS cells using p90Rsk/RSK1/RPS6KA1 Rabbit pAb (A15718) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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