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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | RUN and SH3 domain containing 1 |
| Gene Symbol: | RUSC1 |
| Synonyms: | NESCA; RUSC1 |
| Gene ID: | 23623 |
| UniProt ID: | Q9BVN2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-320 of human RUSC1 (NP_055143.2). |
| Dilution: | WB 1:200-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.57 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-RUSC1 antibody |
Background
Predicted to enable actin binding activity. Involved in protein polyubiquitination. Located in cytosol.
Images
 | Western blot analysis of various lysates using RUSC1 Rabbit pAb (A15417) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunofluorescence analysis of paraffin-embedded mouse brain using RUSC1 Rabbit pAb (A15417) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of paraffin-embedded mouse brain using RUSC1 Rabbit pAb (A15417) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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