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| Reactivity: | Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | sodium voltage-gated channel beta subunit 3 |
| Gene Symbol: | SCN3B |
| Synonyms: | SCNB3; ATFB16; BRGDA7; HSA243396; SCN3B |
| Gene ID: | 55800 |
| UniProt ID: | Q9NY72 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 23-160 of human SCN3B (NP_060870.1). |
| Dilution: | WB 1:2000-1:8000; IF/IC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 7.39 mg/ml |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SCN3B antibody |
Background
Voltage-gated sodium channels are transmembrane glycoprotein complexes composed of a large alpha subunit and one or more regulatory beta subunits. They are responsible for the generation and propagation of action potentials in neurons and muscle. This gene encodes one member of the sodium channel beta subunit gene family, and influences the inactivation kinetics of the sodium channel. Two alternatively spliced variants, encoding the same protein, have been identified.
Images
 | Western blot analysis of lysates from Rat brain, using SCN3B Rabbit pAb (A10316) at 1:7000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of Neuro-2a cells using SCN3B Rabbit pAb (A10316) at dilution of 1:500 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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