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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | succinate dehydrogenase complex flavoprotein subunit A |
| Gene Symbol: | SDHA |
| Synonyms: | FP; PGL5; SDH1; SDH2; SDHF; CMD1GG; MC2DN1; NDAXOA; SDHA |
| Gene ID: | 6389 |
| UniProt ID: | P31040 |
| Clone ID: | 0Y8X5 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 550-650 of human SDHA (P31040). |
| Dilution: | WB 1:1000-1:6000; IF/IC 1:200-1:800 |
| Purification Method: | Affinity purification |
| Concentration: | 0.5 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-SDHA antibody |
Background
This gene encodes a major catalytic subunit of succinate-ubiquinone oxidoreductase, a complex of the mitochondrial respiratory chain. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. Mutations in this gene have been associated with a form of mitochondrial respiratory chain deficiency known as Leigh Syndrome. A pseudogene has been identified on chromosome 3q29. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blot analysis of various lysates using SDHA Rabbit mAb (A13852) at 1：1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Confocal imaging of paraffin-embedded Human colon cancer tissue using SDHA Rabbit mAb (A13852, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 缓冲液体系 prior to IF staining. Objective: 40x. |
 | Confocal imaging of HeLa cells using SDHA Rabbit mAb (A13852, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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