SEC14L2 Rabbit pAb (20 μl)

SEC14L2 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A10586
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IHC, IF/IC, ELISA    
Host Species: Rabbit    
Clonality: Polyclonal antibody    
Gene Name: SEC14 like lipid binding 2
Gene Symbol: SEC14L2
Synonyms: SPF; TAP; TAP1; C22orf6; SEC14L2
Gene ID: 23541
UniProt ID: O76054
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human SEC14L2 (NP_001191133.1).
Dilution: WB 1:1000-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:100
Purification Method: Affinity purification
Concentration: 1.62 mg/ml
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze / thaw cycles.
Documents: Manual-SEC14L2 antibody




Background

This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.




Images

SEC14L2 antibodyWestern blot analysis of various lysates using SEC14L2 Rabbit pAb (A10586) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb (A10586) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb (A10586) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb (A10586) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb (A10586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.



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