Reactivity: | Human, Mouse, Rat |
Applications: | WB, IHC, IF/IC, ELISA |
Host Species: | Rabbit |
Clonality: | Polyclonal antibody |
Gene Name: | SEC14 like lipid binding 2 |
Gene Symbol: | SEC14L2 |
Synonyms: | SPF; TAP; TAP1; C22orf6; SEC14L2 |
Gene ID: | 23541 |
UniProt ID: | O76054 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 60-270 of human SEC14L2 (NP_001191133.1). |
Dilution: | WB 1:1000-1:2000; IHC 1:50-1:200; IF/IC 1:50-1:100 |
Purification Method: | Affinity purification |
Concentration: | 1.62 mg/ml |
Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-SEC14L2 antibody |
Background
This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
Images
![]() | Western blot analysis of various lysates using SEC14L2 Rabbit pAb (A10586) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
![]() | Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using SEC14L2 Rabbit pAb (A10586) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Mouse pancreas using SEC14L2 Rabbit pAb (A10586) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunohistochemistry analysis of paraffin-embedded Rat lung using SEC14L2 Rabbit pAb (A10586) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
![]() | Immunofluorescence analysis of U-2 OS cells using SEC14L2 Rabbit pAb (A10586) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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