Alpha-1 Antitrypsin (SERPINA1) Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A1015
Availability: In Stock

$108.00

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Description

Reactivity:	Human, Mouse
Applications:	WB, IHC, IF/IC, ELISA
Host Species:	Rabbit
Isotype:	IgG
Clonality:	Polyclonal antibody
Gene Name:	serpin family A member 1
Gene Symbol:	SERPINA1
Synonyms:	PI; A1A; AAT; PI1; A1AT; nNIF; PRO2275; alpha1AT; Alpha-1 Antitrypsin (SERPINA1)
Gene ID:	5265
UniProt ID:	P01009
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 156-255 of human Alpha-1 Antitrypsin (SERPINA1) (NP_000286.3).
Dilution:	WB 1:100-1:500; IHC 1:50-1:200; IF/IC 1:50-1:200
Purification Method:	Affinity purification
Concentration:	0.92 mg/ml
Buffer:	PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Storage:	Store at -20°C. Avoid freeze / thaw cycles.
Documents:	Manual-SERPINA1 antibody

Background

The protein encoded by this gene is a serine protease inhibitor belonging to the serpin superfamily whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. This protein is produced in the liver, the bone marrow, by lymphocytic and monocytic cells in lymphoid tissue, and by the Paneth cells of the gut. Defects in this gene are associated with chronic obstructive pulmonary disease, emphysema, and chronic liver disease. Several transcript variants encoding the same protein have been found for this gene.

Images

	Western blot analysis of various lysates using Alpha-1 Antitrypsin (SERPINA1) Rabbit pAb (A1015) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Alpha-1 Antitrypsin (SERPINA1) Rabbit pAb (A1015) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.
	Immunofluorescence analysis of HepG2 cells using Alpha-1 Antitrypsin (SERPINA1) Rabbit pAb (A1015) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.