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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | splicing factor 3b subunit 1 |
| Gene Symbol: | SF3B1 |
| Synonyms: | MDS; PRP10; Hsh155; PRPF10; SAP155; SF3b155; SF3B1 |
| Gene ID: | 23451 |
| UniProt ID: | O75533 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-144 of human SF3B1 (NP_001005526.1). |
| Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 0.91 mg/ml |
| Buffer: | Buffer: PBS with 0.01% thimerosal,50% glycerol,pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
| Documents: | Manual-SF3B1 antibody |
Background
This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blot analysis of various lysates using SF3B1 Rabbit pAb (A15801) at 1:1000 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s. |
 | Immunofluorescence analysis of C6 using SF3B1 Rabbit pAb (A15801) at dilution of d100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 using SF3B1 Rabbit pAb (A15801) at dilution of d100 (40x lens). Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of U-2OS using SF3B1 Rabbit pAb (A15801) at dilution of d100 (40x lens). Blue: DAPI for nuclear staining. |
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