Reactivity: | Human, Mouse, Rat |
Applications: | WB, IF/IC, ELISA |
Host Species: | Rabbit |
Isotype: | IgG |
Clonality: | Polyclonal antibody |
Gene Name: | solute carrier family 25 member 1 |
Gene Symbol: | SLC25A1 |
Synonyms: | CIC; CTP; SEA; CMS23; D2L2AD; SLC20A3; SLC25A1 |
Gene ID: | 6576 |
UniProt ID: | P53007 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-311 of human SLC25A1 (NP_005975.1). |
Dilution: | WB 1:500-1:2000; IF/IC 1:50-1:200 |
Purification Method: | Affinity purification |
Concentration: | 0.56 mg/ml |
Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
Documents: | Manual-SLC25A1 antibody |
Background
This gene encodes a member of the mitochondrial carrier subfamily of solute carrier proteins. Members of this family include nuclear-encoded transporters that translocate small metabolites across the mitochondrial membrane. This protein regulates the movement of citrate across the inner membranes of the mitochondria. Mutations in this gene have been associated with combined D-2- and L-2-hydroxyglutaric aciduria. Pseudogenes of this gene have been identified on chromosomes 7, 11, 16, and 19. Alternative splicing results in multiple transcript variants.
Images
![]() | Western blot analysis of various lysates using SLC25A1 Rabbit pAb (A10247) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
![]() | Immunofluorescence analysis of U2OS cells using SLC25A1 Rabbit pAb (A10247) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of L929 cells using SLC25A1 Rabbit pAb (A10247) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
![]() | Immunofluorescence analysis of C6 cells using SLC25A1 Rabbit pAb (A10247) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining. |
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