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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Polyclonal antibody |
| Gene Name: | SMAD family member 5 |
| Gene Symbol: | SMAD5 |
| Synonyms: | DWFC; JV5-1; MADH5; Smad5 |
| Gene ID: | 4090 |
| UniProt ID: | Q99717 |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 140-240 of human Smad5 (NP_005894.3). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:100 |
| Purification Method: | Affinity purification |
| Concentration: | 1.22 mg/ml |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SMAD5 antibody |
Background
The protein encoded by this gene is involved in the transforming growth factor beta signaling pathway that results in an inhibition of the proliferation of hematopoietic progenitor cells. The encoded protein is activated by bone morphogenetic proteins type 1 receptor kinase, and may be involved in cancer. Alternative splicing results in multiple transcript variants.
Images
 | Western blot analysis of lysates from Rat lung, using Smad5 Rabbit pAb (A0635) at 1:500 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse brain using Smad5 Rabbit pAb (A0635) (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat brain using Smad5 Rabbit pAb (A0635) (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human esophagus using Smad5 Rabbit pAb (A0635) (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining. |
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