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| Reactivity: | Human |
| Applications: | WB, ELISA |
| Host Species: | Rabbit |
| Clonality: | Polyclonal antibody |
| Gene Name: | SPC25 component of NDC80 kinetochore complex |
| Gene Symbol: | SPC25 |
| Synonyms: | AD024; SPBC25; hSpc25; SPC25 |
| Gene ID: | 57405 |
| UniProt ID: | Q9HBM1 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-224 of human SPC25 (NP_065726.1). |
| Dilution: | WB 1:500-1:1000 |
| Purification Method: | Affinity purification |
| Concentration: | 0.86mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SPC25 antibody |
Background
This gene encodes a protein that may be involved in kinetochore-microtubule interaction and spindle checkpoint activity.
Images
 | Western blot analysis of various lysates using SPC25 Rabbit pAb (A10653) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s. |
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