| Reactivity: | M,R & H,M,R | 
| Applications: | WB & IHC | 
| Host Species: | Rabbit | 
| Clonality: | Polyclonal | 
| Full Name: | SUCLG1 rabbit polyclonal  | 
Gene Name:  | Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial  | 
Synonyms:  | Succinyl-CoA synthetase subunit alpha, SCS-alpha, Suclg1, SCS-alpha, GALPHA  | 
Immunogen:  | Recombinant protein corresponding to Mouse SUCLG1  | 
Isotype:  | IgG  | 
Purity:  | Affinity purification  | 
Subcellular location:  | Mitochondrion  | 
Predicted MW.  | 36 kDa  | 
Observed MW.  | 36 kDa  | 
Uniprot ID:  | 
Product Usage Information
Applications  | Species  | Dilution  | Positive tissue  | 
WB  | Mouse, Rat  | 1: 5000-1: 10000  | brain, cerebellum, stomach  | 
IHC  | Human, Mouse, Rat  | 1: 2000-1: 4000  | colon, stomach, small intestine, substantia nigra  | 
Background
SUCLG1(succinyl-CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial) is also named as SCS-alpha and belongs to the succinate/malate CoA ligase alpha subunit family. The SUCLG1 gene encodes a 35 kDa protein with a 40 amino acids transit peptide. This enzyme catalyzes the ATP- or GTP-dependent ligation of succinate and CoA to form succinyl-CoA. Defects in SUCLG1 are the cause of mitochondrial DNA depletion syndrome type 9 (MTDPS9).
Images
  | Western blot analysis of SUCLG1 (GB112774) at dilution of 1: 10000 Lane 1: NIH3T3 cell lysate Lane 2: Mouse brain tissue lysate Lane 3: Mouse cerebellum tissue lysate Lane 4: Mouse stomach tissue lysate Lane 5: Rat brain tissue lysate Lane 6: Rat cerebellum tissue lysate Lane 7: Rat stomach tissue lysate  | 
  | Immunohistochemistry analysis of paraffin-embedded human colon using SUCLG1 (GB112774) at dilution of 1: 4000  | 
  | Immunohistochemistry analysis of paraffin-embedded rat colon using SUCLG1 (GB112774) at dilution of 1: 4000  | 
  | Immunohistochemistry analysis of paraffin-embedded human stomach using SUCLG1 (GB112774) at dilution of 1: 4000  | 
  | Immunohistochemistry analysis of paraffin-embedded mouse small intestine using SUCLG1 (GB112774) at dilution of 1: 4000 | 
  | Immunohistochemistry analysis of paraffin-embedded rat tsubstantia nigra using SUCLG1 (GB112774) at dilution of 1: 4000 | 
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. | 
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |