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| Applications: | WB, IHC, IF/IC, ELISA |
| Host Species: | Mouse |
| Isotype: | IgG1,Kappa |
| Conjugation: | Unconjugated |
| Gene Name: | actin beta |
| Gene Symbol: | ACTB |
| Synonyms: | BRWS1; PS1TP5BP1; β-Actin |
| Gene ID: | 60 |
| UniProt ID: | P60709 |
| Clone ID: | 3W0O2 |
| Immunogen: | Fusion protein of human β-Actin |
| Dilution: | WB 1:5000-1:160000; IHC 1:50-1:200; IF/IC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.757 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual |
Images
 | Western blot analysis of lysates from HeLa cells, using β-Actin Mouse mAb (AC004) at 1:2000- 1:640000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Western blot analysis of various lysates, using β-Actin Mouse mAb (AC004) at 1:20000 dilution. Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using β-Actin Mouse mAb (AC004) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
