Anti-4EBP1 Rabbit pAb (100 μl)

Eukaryotic translation initiation factor 4E-binding protein 1

Synonyms:

4E BP1, 4EBP1, BP 1, eIF4E binding protein 1, EIF4EBP1, PHAS I

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human 4EBP1

Uniprot ID:

Q13541

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Nucleus, Cytoplasm

Predicted MW. / Observed MW.

12.5 kDa / 17 kDa

WB

Human

1: 1000-1: 2000

HeLa,Hep G2,MCF7

IHC/IF

Human

1: 800-1: 1600

breast cancer,colon cancer

WB analysis of 4EBP1 (GB115606).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1:2000, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.

IHC analysis of 4EBP1(GB115606).
Sample: Human breast cancer,4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1:800, 4°Covernight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.

IHC analysis of 4EBP1(GB115606).
Sample: Human colon cancer,4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1:800, 4°C overnight.
Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h.