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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Astacin-like metalloendopeptidase |
Synonyms: | ASTL, Oocyte astacin, Ovastacin, SAS1B |
Immunogen: | Recombinant protein corresponding to Mouse ASTL |
Uniprot ID: | Q6HA09 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 47 kDa / 47 kDa |
Product Usage Information
WB | Human | 1: 2000-1: 3000 | MCF7 |
Background
Oocyte-specific oolemmal receptor involved in sperm and egg adhesion and fertilization. Plays a role in the polyspermy inhibition. Probably acts as a protease for the post-fertilization cleavage of ZP2. Cleaves the sperm-binding ZP2 at the surface of the zona pellucida after fertilization and cortical granule exocytosis, rendering the zona pellucida unable to support further sperm binding.
Images
| Western blot analysis of ASTL (GB115383) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |