| Reactivity: | M,R | 
| Applications: | WB | 
| Host Species: | Rabbit | 
| Clonality: | Polyclonal | 
| Gene Name: | ATPase inhibitor, mitochondrial  | 
Synonyms:  | Atpi, Atpif1  | 
Immunogen:  | Recombinant protein corresponding to Mouse ATP5IF1  | 
Uniprot ID:  | O35143  | 
Isotype:  | IgG  | 
Purity:  | Affinity purification  | 
Predicted MW. / Observed MW.  | 12 kDa / 14 kDa  | 
Product Usage Information
WB  | Mouse, Rat  | 1: 300-1: 800  | kidney  | 
Background
Endogenous F(1)F(o)-ATPase inhibitor limiting ATP depletion when the mitochondrial membrane potential falls below a threshold and the F(1)F(o)-ATP synthase starts hydrolyzing ATP to pump protons out of the mitochondrial matrix.Required to avoid the consumption of cellular ATP when the F(1)F(o)-ATP synthase enzyme acts as an ATP hydrolase.Indirectly acts as a regulator of heme synthesis in erythroid tissues: regulates heme synthesis by modulating the mitochondrial pH and redox potential, allowing FECH to efficiently catalyze the incorporation of iron into protoporphyrin IX to produce heme.
Images
  | WB analysis of ATP5IF1(GB115625).  | 
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. | 
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |