| Reactivity: | Mouse, Rat |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | POU domain, class 4, transcription factor 1 |
| Gene Symbol: | Pou4f1 |
| Synonyms: | Brn3; Brn-3; Brn3a; Brn3.0; Brn-3.0; E130119J07Rik |
| Gene ID: | 18996 |
| Uniprot ID: | P17208, P20266 |
| Immunogen: | Recombinant protein corresponding to Mouse Pou4f1 |
| Isotype: | IgG |
| Purity: | Affinity purification |
| Subcellular location: | Nucleus |
| Documents: | Manual-POU4F1 Antibody |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 400-1: 1400 | brain, embryonic mouse, embryonic rat, epencephalon, eye |
Background
Enables several functions, including DNA binding activity; DNA-binding transcription factor activity, RNA polymerase II-specific; and GTPase binding activity. Involved in several processes, including cellular response to estradiol stimulus; nervous system development; and regulation of gene expression. Acts upstream of or within several processes, including nervous system development; proprioception involved in equilibrioception; and suckling behavior. Located in several cellular components, including chromatin; neuron projection; and nucleus. Part of RNA polymerase II transcription regulator complex. Is expressed in several structures, including branchial arch mesenchyme; central nervous system; future brain; ganglia; and sensory organ. Orthologous to human POU4F1 (POU class 4 homeobox 1).
Images
![]() | Immunohistochemistry of paraffin embedded mouse brain using BRN3A (GB111661) at dilution of 1:700 (400x lens) |
![]() | Immunohistochemistry of paraffin embedded mouse embryonic mouse using BRN3A (GB111661) at dilution of 1:700 (400x lens) |
![]() | Immunohistochemistry of paraffin embedded rat brain using BRN3A (GB111661) at dilution of 1:700 (400x lens) |
![]() | Immunohistochemistry of paraffin embedded rat embryonic rat using BRN3A (GB111661) at dilution of 1:700 (400x lens) |
![]() | Immunofluorescence of paraffin embedded mouse brain using BRN3A (GB111661) at dilution of 1:650 (400x lens) |
![]() | Immunofluorescence of paraffin embedded rat embryonic rat using BRN3A (GB111661) at dilution of 1:650 (400x lens) |
![]() | Immunofluorescence of paraffin embedded rat epencephalon using BRN3A (GB111661) at dilution of 1:650 (400x lens) |
![]() | Immunofluorescence of paraffin embedded rat eye using BRN3A (GB111661) at dilution of 1:650 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
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