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Anti-CD11b Rabbit pAb (100 μl)
| Reactivity: | M & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Integrin alpha-M |
Synonyms: | CD11B, CR 3 alpha chain, CR3A, ITGAM, MAC 1, MAC1A, MO1A, Neutrophil adherence receptor, SLEB6 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CD11b |
Uniprot ID: | P05555 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 127 kDa / 170 kDa |
Product Usage Information
WB | Mouse | 1: 250-1: 750 | RAW264.7, spleen+LPS |
IHC/IF | Mouse | 1: 500-1: 1000 | brain from MACO model, Inflammatory model induced by lps(24h) liver |
Background
Integrin ITGAM/ITGB2 is implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles and pathogens (By similarity).It is identical with CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the R-G-D peptide in C3b. Integrin ITGAM/ITGB2 is also a receptor for fibrinogen, factor X and ICAM1. It recognizes P1 and P2 peptides of fibrinogen gamma chain. Regulates neutrophil migration. In association with beta subunit ITGB2/CD18, required for CD177-PRTN3-mediated activation of TNF primed neutrophils (By similarity).May regulate phagocytosis-induced apoptosis in extravasated neutrophils (By similarity).May play a role in mast cell development (By similarity).
Images
| WB analysis of CD11b(GB115689). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of CD11b(GB115689). Sample: Mouse brain from MACO model, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD11b(GB115689). Sample: Mouse Inflammatory model induced by lps(6h) liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD11b(GB115689). Sample: Mouse Inflammatory model induced by lps(24h) lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD11b(GB115689). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IF analysis of CD11b(GB115689) red. Sample: Mouse brain from MACO model, 4% PFA (G1101) 12-24h. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IF analysis of CD11b(GB115689) red. Sample: Mouse Inflammatory model induced by lps(24h) lung, 4% PFA (G1101) 12-24h. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IF analysis of CD11b (GB115689)red. sample: Mouse Inflammatory model induced by lps(24h) liver, 4% PFA (G1101) 12-24h. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD11b (GB115689) red 1: 2500, 4°C overnight.HRP Goat Anti-Rabbit lgG (GB23303)+cy3-TSA(G1223). CD68 (GB113109) green 1: 200,4°C overnight, 488 Goat Anti-Rabbit lgG(GB25303). |
| IF analysis of CD11b (GB115689)red. sample: Mouse Inflammatory model induced by lps(24h) lung, 4% PFA (G1101) 12-24h. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: CD11b (GB115689)red 1: 2500, 4°C overnight.HRP Goat Anti-Rabbit lgG (GB23303)+cy3-TSA(G1223). CD68 (GB113109) green 1: 200,4°C overnight, 488 Goat Anti-Rabbit lgG（GB25303）. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |