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| Reactivity: | M & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Tumor necrosis factor receptor superfamily member 5 |
Synonyms: | CD40, Bp50, CDW40, TNFRSF5, p50, CD40 (protein), CD40 molecule, B cell-associated molecule, TNF receptor superfamily member 5 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CD40 |
Uniprot ID: | P27512 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 31 kDa / 40-50 kDa |
Product Usage Information
WB | Mouse | 1: 300-1: 500 | spleen,lymph node |
IHC/IF | Mouse | 1: 500-1: 1000 | Inflammatory model induced by lps(6h) spleen, lymph node |
Background
Receptor for TNFSF5/CD40LG (By similarity).
Transduces TRAF6- and MAP3K8-mediated signals that activate ERK in macrophages and B cells, leading to induction of immunoglobulin secretion.
Images
| WB analysis of CD40(GB115671). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| WB analysis of CD40(GB115671). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of CD40(GB115671). Sample: Mouse Inflammatory model induced by lps(6h) spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody:HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD40(GB115671). Sample: Mouse Inflammatory model induced by lps(6h) spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody:HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD40(GB115671). Sample: Mouse lymph node, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody:HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD40(GB115671). Sample: Mouse spleen, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody:HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |