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| Reactivity: | H & H & H |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | CD63 antigen |
Synonyms: | CD63, LAMP-3, ME491, MLA1, OMA81H, TSPAN30, CD63 molecule, Lysosomal associated membrane protein 3, LAMP3, CD63 antigen, NGA, Tetraspanin-30 |
Immunogen: | Recombinant protein corresponding to Human CD63 |
Uniprot ID: | P08962 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, late endosome, lysosome, exosome |
Predicted MW. / Observed MW. | 25 kDa / 25-60 kDa |
Applications
WB | Human | 1: 300-1: 500 | A-375, SW1990-exo, malignant melanoma |
IHC/IF | Human | 1:1500-1:3000 | ovarian cancer |
ICC/IF | Human | 1: 1500-1: 3000 | HeLa |
Background
CD63 antigen is a protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth, and motility. CD63 is a cell surface glycoprotein that is known to complex with integrins.CD63 is mainly associated with membranes of intracellular vesicles, although cell surface expression may be induced.
Images
| WB analysis of CD63(GB115694). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of CD63(GB115694) red. Sample: A431, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of CD63(GB115694) red. Sample: HeLa, 4% PFA (G1101) 20min. Blocking buffer: Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| IHC analysis of CD63(GB115694). Sample: Human liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min. |
| IHC analysis of CD63(GB115694). Sample: Human ovarian cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L), Ready to use(G1302), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.