Anti-CD69 Rabbit pAb (100 μl)

Early activation antigen CD69

Synonyms:

Activation inducer molecule, AIM, BL AC/P26, CD69, CD69 molecule, CLEC2C, EA1, Early activation antigen CD69, GP32/28, MLR 3

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human CD69

Uniprot ID:

Q07108

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Cell membrane

Predicted MW. / Observed MW.

23 kDa / 28/32 kDa

WB

Human

1: 300-1: 1000

Molt-4, Jurkat

IHC/IF

Human

1: 700-1: 1400

cervical carcinoma, tonsil

WB analysis of CD69(GB115670).

Sample: Protein treated by RIPA Lysis Buffer (G2002).

Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.

Primary antibody: 1: 500, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

IHC analysis of CD69(GB115670).

Sample: Human tonsil, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 700, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of CD69(GB115670).

Sample: Human tonsil, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 700, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of CD69(GB115670).

Sample: Human cervical carcinoma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of CD69(GB115670).

Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.

Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min.

Primary antibody: 1: 1000, 4°C overnight.

Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.