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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Serine/ threonine-protein kinase Chk2 |
Synonyms: | CHEK2, CDS1, CHK2, HuCds1, LFS2, PP1425, RAD53, hCds1, Checkpoint kinase 2 |
Immunogen: | Recombinant protein corresponding to Mouse Chk2 |
Uniprot ID: | Q9Z265 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 61 kDa / 61 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | kidney, thymus |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | colon |
Background
CHEK2 (Checkpoint kinase 2) is a tumor suppressor gene that encodes the protein CHK2. CHK2 is mainly involved in DNA repair, cell cycle arrest or apoptosis in response to DNA damage. When DNA undergoes a double-strand break, CHK2 is activated. Specifically, DNA damage-activated phosphatidylinositol kinase family protein (PIKK) ATM phosphorylates site Thr68 and activates CHK2. Once activated, CHK2 phosphorylates downstream targets including CDC25 phosphatases, responsible for dephosphorylating and activating the cyclin-dependent kinases (CDKs).
Images
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Western blot analysis of Chk2 (GB11575) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded rat colon using Chk2 (GB11575) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded mouse colon using Chk2 (GB11575) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human colon using Chk2 (GB11575) at dilution of 1: 1000 |
Storage
| Storage |
Store at -20°C for one year. Avoid repeated freeze/thaw cycles |
| Storage Buffer |
PBS with 0.02%sodium azide, 100 μg/ml BSA and 50% glycerol. |