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Anti-Collagen III Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Collagen alpha-1(III) chain |
Synonyms: | COL3A1, EDS4A, collagen type III alpha 1, collagen type III alpha 1 chain |
Immunogen: | Recombinant protein corresponding to Mouse Collagen III |
Uniprot ID: | P08121 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Predicted MW. / Observed MW. | 138 kDa / 140-220 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 500-1: 1000 | skin |
IHC/IF | Human, Mouse, Rat | 1: 250-1: 1000 | kidney, liver, lung |
Background
The gene COL3A1 encodes the pro-alpha1 chains of type III collagen, a fibrillar collagen that is found in extensible connective tissues such as skin, lung, uterus, intestine and the vascular system, frequently in association with type I collagen. Mutations in this gene are associated with Ehlers-Danlos syndrome types IV, and with aortic and arterial aneurysms. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
Images
| WB analysis of collagen III (GB111629). Sample: Protein treated by RIPA Lysis Buffer (G2002),☆ sample of skin treated by RIPA+SDS Lysis Buffer. Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of collagen III (GB111629). Sample: Human kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of collagen III (GB111629). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of collagen III (GB111629). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IF analysis of collagen III (GB111629). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of collagen III (GB111629). Sample: Mouse lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of collagen III (GB111629). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of collagen III (GB111629). Sample: Rat lung (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Pepsin Antigen Repair Solution,37°C,30min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
