Anti-Collagen IV Rabbit pAb (100 μl)

Collagen alpha-1(IV) chain

Synonyms:

COL4A1

Immunogen:

KLH conjugated Synthetic peptide corresponding to Human Collagen alpha-1(IV)

Uniprot ID:

P02462

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Secreted, extracellular space, extracellular matrix, Basement membrane

Predicted MW. / Observed MW.

160 kDa / 200-220 kDa

WB

Human

1: 300-1: 500

HS578T, HT1080

IHC/IF

Human

1: 1000-1: 2000

kidney, liver cancer

WB analysis of Collagen IV(GB115688).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

IHC analysis of collagen IV(GB115688).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Collagen IV(GB115688).
Sample: Human liver cancer, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.