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Anti-Cyclin D1 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | G1/S-specific cyclin-D1 |
Synonyms: | Ccnd1, Cyl-1, B-cell lymphoma 1 protein, BCL-1, Parathyroid adenomatosis 1, PRAD1, BCL1, CCND1, D11S287E, U21B31, cyclin D1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cyclin D1 |
Uniprot ID: | P25322 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 33 kDa / 33 kDa |
Applications
WB | Human, Mouse, Rat | 1: 300-1: 800 | MCF7, HeLa, LnCap |
IHC/IF | Mouse, Rat | 1: 600-1: 1200 | skin, esophagus, colon |
Background
The protein belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb.
Images
| Western blot analysis of Cyclin D1 (GB111372)at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat colon using Cyclin D1 (GB111372) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin-embedded mouse skin using Cyclin D1 (GB111372) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin-embedded mouse esophagus using Cyclin D1 (GB111372) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin-embedded mouse colon using Cyclin D1 (GB111372) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin-embedded rat colon using Cyclin D1 (GB111372) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin-embedded mouse stomach using Cyclin D1 (GB111372) at dilution of 1: 600 |
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Immunohistochemistry analysis of paraffin-embedded rat stomach using Cyclin D1 (GB111372) at dilution of 1: 600 |
 | Immunofluorescence analysis of paraffin embedded rat colon using Cyclin D1 (GB111372) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |