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Anti-Elongin A Rabbit pAb (100 μl)
| Reactivity: | H & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Elongin-A |
Synonyms: | EloA, Elongin 110 kDa subunit, RNA polymerase II transcription factor SIII subunit A1, SIII p110, Transcription elongation factor B polypeptide 3, Tceb3, |
Immunogen: | Recombinant protein corresponding to Mouse Elongin A |
Uniprot ID: | Q8CB77 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 87 kDa / 105 kDa |
Product Usage Information
WB | Human | 1: 1000-1: 2000 | HUVEC, Jurkat, HeLa, 293, A-431 |
IHC/IF | Mouse | 1: 1500-1: 3000 | cerebellum, kidney |
Background
SIII, also known as elongin, is a general transcription elongation factor that increases the RNA polymerase II transcription elongation past template-encoded arresting sites. Subunit A is transcriptionally active and its transcription activity is strongly enhanced by binding to the dimeric complex of the SIII regulatory subunits B and C (elongin BC complex).
Images
| Western blot analysis of ELOA1 (GB114135) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse cerebellum using ELOA1 (GB114135) at dilution of 1: 3000 |
| Immunofluorescence analysis of paraffin embedded mouse kidney using ELOA1 (GB114135) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |