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Anti-FACL4 Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Long-chain-fatty-acid--CoA ligase 4 |
Synonyms: | ACS4, FACL4, LACS4 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human FACL4 |
Uniprot ID: | O60488 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 79 kDa / 79 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 2000 | Hep G2,Huh7,RAW 264.7,liver |
Background
Catalyzes the conversion of long-chain fatty acids to their active form acyl-CoA for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially activates arachidonate and eicosapentaenoate as substrates. Preferentially activates 8,9-EET > 14,15-EET > 5,6-EET > 11,12-EET. Modulates glucose-stimulated insulin secretion by regulating the levels of unesterified EETs (By similarity). Modulates prostaglandin E2 secretion.
Images
| WB analysis of FACL4 (GB115608). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |