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Anti-Integrin beta 1/CD29 Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Integrin beta-1 |
Synonyms: | CD29, FNRB, GPIIA, Integrin beta 1, ITB1, ITGB1, MDF2, MSK12, VLA 4 subunit beta, VLA BETA, VLAB |
Immunogen: | KLH conjugated Synthetic peptide corresponding to mouse CD29 |
Uniprot ID: | P09055 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 88 kDa / 120-150 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | kidney,liver,spleen,heart |
IHC/IF | Mouse, Rat | 1: 500-1: 1000 | colon, kidney |
Background
Integrins alpha-1/beta-1, alpha-2/beta-1, alpha-10/beta-1 and alpha-11/beta-1 are receptors for collagen.Integrins alpha-1/beta-1 and alpha-2/beta-2 recognize the proline-hydroxylated sequence G-F-P-G-E-R in collagen.Integrins alpha-2/beta-1, alpha-3/beta-1, alpha-4/beta-1, alpha-5/beta-1, alpha-8/beta-1, alpha-10/beta-1, alpha-11/beta-1 and alpha-V/beta-1 are receptors for fibronectin.Alpha-4/beta-1 recognizes one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin.Integrin alpha-5/beta-1 is a receptor for fibrinogen.Integrin alpha-1/beta-1, alpha-2/beta-1, alpha-6/beta-1 and alpha-7/beta-1 are receptors for lamimin.Integrin alpha-6/beta-1 (ITGA6:ITGB1) is present in oocytes and is involved in sperm-egg fusion.
Images
| WB analysis of CD29(GB115629). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of CD29(GB115629). Sample:Mouse colon ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD29(GB115629). Sample:Mouse kidney ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD29(GB115629). Sample:Mouse liver ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD29(GB115629). Sample:Rat colon ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD29(GB115629). Sample:Rat kidney ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of CD29(GB115629). Sample:Rat liver ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min.Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |