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| Reactivity: | M,R & M,R |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MED3 rabbit polyclonal |
Gene Name: | Mediator of RNA polymerase II transcription subunit 27 |
Synonyms: | Cofactor required for Sp1 transcriptional activation subunit 8, CRSP complex subunit 8, Mediator complex subunit 27, Med27, Crsp8 |
Immunogen: | Recombinant protein corresponding to Mouse MED3 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 3600-1: 7200 | testis, hippocampus |
IF | Mouse, Rat | 1: 500-1: 1000 | testis, hippocampus |
Background
Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Images
| Immunohistochemistry analysis of paraffin-embedded mouse testis using MED3 (GB112741) at dilution of 1: 7200 |
| Immunohistochemistry analysis of paraffin-embedded rat testis using MED3 (GB112741) at dilution of 1: 7200 |
| Immunohistochemistry analysis of paraffin-embedded mouse hippocampus using MED3 (GB112741) at dilution of 1: 7200 |
| Immunohistochemistry analysis of paraffin-embedded rat hippocampus using MED3 (GB112741) at dilution of 1: 7200 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using MED3 (GB112741) at dilution of 1: 1000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat hippocampus using MED3 (GB112741) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |