Your shopping cart is empty!
| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Mixed lineage kinase domain-like protein |
Synonyms: | hMLKL, Mixed lineage kinase domain like, Mixed lineage kinase domain like protein, Mixed lineage kinase domain-like protein, mixed lineage kinase domain like pseudokinase |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human MLKL |
Uniprot ID: | Q8NB16 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Cell membrane, Nucleus |
Predicted MW. / Observed MW. | 54 kDa / 54 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1: 500-1: 1000 | HeLa, HT-29, PC-3 |
IHC/IF | Human | 1: 500-1: 1000 | liver cancer, tonsil |
Background
This gene belongs to the protein kinase superfamily. The encoded protein contains a protein kinase-like domain; however, is thought to be inactive because it lacks several residues required for activity. This protein plays a critical role in tumor necrosis factor (TNF)-induced necroptosis, a programmed cell death process, via interaction with receptor-interacting protein 3 (RIP3), which is a key signaling molecule in necroptosis pathway. Inhibitor studies and knockdown of this gene inhibited TNF-induced necrosis. High levels of this protein and RIP3 are associated with inflammatory bowel disease in children. Alternatively spliced transcript variants have been described for this gene.
Images
| WB analysis of MLKL（GB115699). |
| IF analysis of MLKL(GB115699) red. |
| IF analysis of MLKL(GB115699) red. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |