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Anti-MRPL12 Rabbit pAb (100 μl)
| Reactivity: | R & H,M,R |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 39S ribosomal protein L12, mitochondrial |
Synonyms: | L12mt, MRP-L12, Rpml12, Mrpl12, 5c5-2, MRPL7 |
Immunogen: | Recombinant protein corresponding to Mouse MRPL12 |
Uniprot ID: | Q9DB15 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion |
Product Usage Information
IHC/IF | Rat | 1: 1800-1: 3600 | colon |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | HeLa |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.
Images
| Immunohistochemistry of paraffin embedded rat colon using Mrpl12 (GB112124) at dilution of 1:1600 (400x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using MRPL12(GB112124) at dilution of 1:100-1:400 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using MRPL12(GB112124) at dilution of 1:100-1:400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |