| Reactivity: | M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 5 |
Synonyms: | B13, CI 13KD B, Complex I 13kD B, Complex I subunit B13, NDUFA5, NUFM, UQOR13 |
Immunogen: | Recombinant protein corresponding to Mouse NDUFA5 |
Uniprot ID: | Q9CPP6 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 13 kDa / 13 kDa |
Product Usage Information
WB | Mouse, Rat | 1:3000-1:5000 | heart, brain, liver |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
Accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I), that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
Images
| Western blot analysis of NDUFA5 (GB114241) at dilution of 1: 3000 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using NDUFA5(GB114241) at dilution of 1: 50-1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |