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Anti-PARIS Rabbit pAb (100 μl)
| Reactivity: | H & M,R & M,R |
| Applications: | WB & IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | PARIS rabbit polyclonal |
Gene Name: | Zinc finger protein 746 |
Synonyms: | Znf746, Zfp746, PARIS, Parkin-interacting substrate |
Immunogen: | Recombinant protein corresponding to Mouse PARIS |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human | 1: 500-1: 1000 | THP-1, SH-SY5Y, U251 |
IHC | Mouse, Rat | 1: 1500-1: 3000 | testis, substantia nigra |
IF | Mouse, Rat | 1: 1500-1: 3000 | testis, frontal lobe |
Background
ZNF746, also named as PARIS, which belongs to the krueppel C2H2-type zinc-finger protein family. ZNF746 mainly localizes to the cytoplasm and probably translocates to the nucleus to repress selected genes. ZNF746 as a transcription repressor that specifically binds to the 5'-TATTTT[T/G]-3' consensus sequence on promoters and repress transcription of PGC-1-alpha (PPARGC1A), thereby playing a role in regulation of neuron death. ZNF746 may act as a downstream effector of PRKN and contribute to neurodegeneration in Parkinson disease cases caused by defects in PRKN.
Images
| Western blot analysis of PARIS (GB112763) at dilution of 1: 1000 Lane 1: THP-1 cell lysate Lane 2: SH-SY5Y cell lysate Lane 3: U251 cell lysate |
| Immunohistochemistry analysis of paraffin-embedded mouse testis using PARIS (GB112763) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat testis using PARIS (GB112763) at dilution of 1: 3000 |
| Immunohistochemistry analysis of paraffin-embedded rat substantia nigra using PARIS (GB112763) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using PARIS (GB112763) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraformaldehyde-fixed rat frontal lobe using PARIS (GB112763) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |