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Anti-Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Phospho-ERK1 (T202/Y204) + ERK2 (T185/Y187) rabbit polyclonal |
Gene Name: | Mitogen-activated protein kinase 3 |
Synonyms: | APK1, ERK, ERK-1, ERK-2, ERK1, ERK2, ERT1, MAPK1,MAPK3, P42MAPK, P44MAPK, PRKM1, PRKM2, p44, p42, p44-MAPK, p42-MAPK, P-ERK, P-ERK1/2, P-ERK1, P-ERK2 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human ERK1 (T202/Y204) + ERK2 (T185/Y187) |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 44/42 kDa |
Observed MW. | 44/42 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | PC12, HT29, NIH/3T3 |
Background
ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to INS and NGF. This antibody can recognize both ERK1 and ERK2 with the molecular mass of 38-44 kDa.
Images
 | Western blot analysis of ERK1 (T202/Y204) +ERK2 (T185/Y187) GB113492 at dilution of 1: 2000 Lane 1: PC12 untreated Lane 2: PC12 treated with UV ( 30min) Lane 3: HT29 untreated Lane 4: HT29 treated with UV ( 30min) Lane 5: NIH/3T3 untreated Lane 6: NIH/3T3 treated with UV (30min) |
| Western blot analysis of ERK1 (T202/Y204) +ERK2 (T185/Y187) GB113492 at dilution of 1: 2000 Lane1: Jurkat untreated, whole cell lysate Lane2: Jurkat was treated with 200 nM TPA for 10min, whole cell lysate Lane3: 293 untreated, whole cell lysate Lane4: 293 was treated with 200 nM TPA for 10min, whole cell lysate |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |