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Anti-Phospho-MEK1/2 (S218/S222) Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Dual specificity mitogen-activated protein kinase kinase 1/2 |
Synonyms: | MAP2K1, MAP2K2, MEK1, PRKMK1，MEK2, MKK2, PRKMK2 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human MEK1/2（S218/222) |
Uniprot ID: | Q02750,P36507 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 43/41kDa（MEK1）44kDa(MEK2) / 45 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 100-1: 300 | HeLa, NIH3T3, C6 |
Background
Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor as well as interleukin (IL)-10 receptor. Directly phosphorylates STAT but also activates STAT signaling through the transactivation of other JAK kinases associated with signaling receptors.
Images
| WB analysis of Phospho-MEK1/2 (S218/222)(GB115603). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1:300, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| WB analysis of Phospho-MEK1/2（S218/S222)(GB115603). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |