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Anti-Phospho-S6 Ribosomal Protein (S235/236) Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Small ribosomal subunit protein eS6 |
Synonyms: | 40S ribosomal protein S6, Cell and organelle markers, Phosphoprotein NP33, Phospho-RPS6 (Ser235/236), Phospho-S6 Ribosomal Protein (Ser235/236), ribosomal protein S6, Ribosome Marker, RPS6, S6 Ribosomal Protein |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human RPS6 (S235/236) |
Uniprot ID: | P62753 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Predicted MW. / Observed MW. | 29 kDa / 32 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 2000-1: 3000 | HeLa,NIH/3T3 |
IHC/IF | Human, Mouse, Rat | 1: 700-1: 1400 | endometrial cancer, ovarian cancer |
Background
Hydrolyzes extracellular nucleotides into membrane permeable nucleosides.
Images
| WB analysis of Phospho-S6 Ribosomal Protein (S235/236)(GB115635). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 2000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein (S235/236)(GB115635). Sample:Human endometrial Cancer ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 700, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein (S235/236)(GB115635). Sample:Human ovarian cancer ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 700, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein (S235/236)(GB115635). Sample:Mouse spleen ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 700, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
| IHC analysis of Phospho-S6 Ribosomal Protein (S235/236)(GB115635). Sample:Rat spleen ，4% PFA (G1101) 12-24h. Antigen retrieval:Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 700, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |