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Anti-Phospho-STAT1 (Y701) Rabbit pAb (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Signal transducer and activator of transcription 1 |
Synonyms: | STAT1 |
Immunogen: | KLH conjugated Synthetic phosphopeptide corresponding to Human STAT1(Tyr701) |
Uniprot ID: | P42224 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 87/83 kDa / 91 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | C2C12+IFN-γ, C6 |
Background
Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2.
Images
| WB analysis of Phospho-STAT1(Y701) (GB115605). |
| WB analysis of Phospho-STAT1(Y701)(GB115605). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |