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| Reactivity: | H,M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Reversion-inducing cysteine-rich protein with Kazal motifs |
Synonyms: | hRECK，ST15 |
Immunogen: | Recombinant protein corresponding to Mouse RECK |
Uniprot ID: | Q9Z0J1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 106 kDa / 106 kDa |
Product Usage Information
WB | Human | 1: 300-1: 600 | MCF7 |
Background
Functions together with ADGRA2 to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) . Plays a key role in Wnt7-specific responses: required for central nervous system (CNS) angiogenesis and blood-brain barrier regulation . Acts as a Wnt7-specific coactivator of canonical Wnt signaling by decoding Wnt ligands: acts by interacting specifically with the disordered linker region of Wnt7, thereby conferring ligand selectivity for Wnt7 (By similarity). ADGRA2 is then required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). Also acts as a serine protease inhibitor: negatively regulates matrix metalloproteinase-9 (MMP9) by suppressing MMP9 secretion and by direct inhibition of its enzymatic activity . Also inhibits metalloproteinase activity of MMP2 and MMP14 (MT1-MMP).
Images
| Western blot analysis of RECK (GB114210) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |