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| Reactivity: | M & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Receptor-interacting serine/threonine-protein kinase 3 |
Synonyms: | Rip3 , KIAA0864, M RIP, MPRIP, MRIP, p116Rip, Rho interacting protein 3, RHOIP3, RIP3 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse RIP3 |
Uniprot ID: | Q9QZL0 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 53 kDa / 53 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Mouse | 1: 500-1: 1000 | L-929,RAW 264.7,N2A(low),A20 |
ICC/IF | Mouse | 1: 500-1: 1000 | L929, NIH-3T3 |
Background
Serine/threonine-protein kinase that activates necroptosis and apoptosis, two parallel forms of cell death.Necroptosis, a programmed cell death process in response to death-inducing TNF-alpha family members, is triggered by RIPK3 following activation by ZBP1.Activated RIPK3 forms a necrosis-inducing complex and mediates phosphorylation of MLKL, promoting MLKL localization to the plasma membrane and execution of programmed necrosis characterized by calcium influx and plasma membrane damage.In addition to TNF-induced necroptosis, necroptosis can also take place in the nucleus in response to orthomyxoviruses infection: following ZBP1 activation, which senses double-stranded Z-RNA structures, nuclear RIPK3 catalyzes phosphorylation and activation of MLKL, promoting disruption of the nuclear envelope and leakage of cellular DNA into the cytosol.
Images
| WB analysis of RIPK3(GB115634). |
| ICC analysis of RIP3(GB115634)red. Sample: L929, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
| ICC analysis of RIP3(GB115634)green. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: 488 conjugated Goat Anti-Rabbit IgG (H+L) (GB25303), 1: 400, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |