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Anti-RPS18 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 40S ribosomal protein S18 |
Synonyms: | Ke-3, HKE3, Ribosomal protein S18, Rhabdomyosarcoma antigen MU-RMS-40.21, Rps18 |
Immunogen: | Recombinantproteincorresponding to Mouse RPS18 |
Uniprot ID: | P62270 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 18 kDa / 18 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, skin, lymph gland |
ICC/IF | Mouse | 1: 100-1; 300 | NIH-3T3 |
Background
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E.
Images
| WB analysis of RPS18(GB111706). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of RPS18(GB111706)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |