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Anti-RUNX2 Rabbit pAb (100 μl)
| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Runt-related transcription factor 2 |
Synonyms: | AML3, CBF alpha 1, CBFA1, CCD, CCD1, Oncogene AML 3, OSF 2, OSF2, PEA2 alpha A, PEA2aA, PEBP2 alpha A, PEBP2A, PEBP2A1, PEBP2A2, PEBP2aA, PEBP2aA1, RUNX2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human RUNX2 |
Uniprot ID: | Q13950 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 57 kDa / 55-62 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
IHC/IF | Mouse Rat | 1: 400-1: 800 | Bone, spleen |
Background
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis.Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification.CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters.In osteoblasts, supports transcription activation: synergizes with SPEN/MINT to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE).Inhibits KAT6B-dependent transcriptional activation.
Images
| IHC analysis of RUNX2(GB115631). Sample: Mouse Bone 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1：500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of RUNX2(GB115631). Sample: Mouse Bone 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of RUNX2(GB115631). Sample: Mouse spleen 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of RUNX2(GB115631). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of RUNX2(GB115631). Sample: Mouse bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of RUNX2(GB115631). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
| IHC analysis of RUNX2(GB115631). Sample: Rat bone, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |